I am currently running Twonky Media 4.4.9 and recently upgraded my iTunes to 8.0.
This upgrade killed my Apple TV, it is no longer recognized by iTunes, but it also seem to have upset my ability to stream my content to my Freecom MusicPal.
Is it possible that iTune somehow causes problems with Twonky's ability to stream files? I don't think its a network problem because the MusicPal can still stearm media from the internet and Twonky sees the devise in the configuration menu.
Any ideas other than waiting for Apple?